Nchịkọta Ọbara Nkewa Gel Tube
Nkọwa dị mkpirikpi:
Ha nwere jel pụrụ iche nke na-ekewa mkpụrụ ndụ ọbara na serum, yana ihe ndị na-eme ka ọbara na-akpụkọ ngwa ngwa. Enwere ike ịmegharị ihe nlele ọbara na centrifuged, na-ekwe ka wepụ serum doro anya maka nyocha.
Mgbe achọrọ mmiri oyi kpọnwụrụ, tinye tube (s) mbufe rọba ozugbo n'ime friza nkefriji. Gwa onye nnọchi anya ọkachamara gị na ị nwere ụdị oyi kpọnwụrụ a ga-ewereelu; A ga-edobe ụdị oyi kpọnwụrụ dị iche maka ule ọ bụla chọrọ ụdị oyi kpọnwụrụ.Ọbara Separator Tubes (SST).Serum separator (ọlaedo, mottled red/isi awọ n'elu) tubes nwere mkpịsịactivator na gel maka ikewapụ serum na sel mana agụnyeghị ọgwụ mgbochi ọrịa. Soro usoro ndị a mgbeiji serum separator tube; Ejila serum separator tubes nyefe ụdị nke tricyclic.A na-arịọ ọkwa antidepressant, Coombs Direct, Group Blood, na Ụdị.
1.Draw dum ọbara na ihe ego 21/2 ugboro a chọrọ olu serum nke mere na a zuru ezu ego.Enwere ike nweta serum. The 5 ml Gold top tube ga-emepụta ihe dị ka 2 mL serum mgbe clotting nacentrifuging.Oghere dị n'elu 10 mL mottled na-acha uhie uhie/acha ntụ ntụ na-emepụta ihe dị ka 4 ml serum. Debe ihe atụ ahụ.dabara adaba.
2. Jiri nwayọọ tụgharịa serum separator tube ugboro ise ka ịgwakọta clot activator na ọbara.
3. Tinye tube nchịkọta ahụ n'ebe kwụ ọtọ na akwa akwa, ma kwe ka ọbara kụọ n'ime ụlọ okpomọkụ.n'ihi na ọ bụghị ihe karịrị 30-45 nkeji. (Mkpịsị na-emekarị na-etolite na 20-30 nkeji.)
4.Mgbe ikwe ka clot na-etolite 20-30 nkeji, tinye tube na centrifuge, nkwụsị nkwụsị.centrifuge maka nkeji iri na ise na ọsọ nke onye nrụpụta tụrụ aro. Ekwela ka ogologo ogecentrifugation n'ihi na nke a nwere ike ịkpata hemolysis. Mgbe ị na-eji bench-top centrifuge, were a itule tube nkeotu ụdị nwere nha nha mmiri.
5. Gbanyụọ centrifuge ma kwe ka ọ bịa na njedebe zuru oke. Ejila aka ma ọ bụ breeki kwụsị ya.Wepu yatube nke ọma na-enweghị imebi ihe dị n'ime ya. Nyochaa gel mgbochi iji hụ na o mechiela ọbara ahụ site nasel ndị juru n'ọnụ. Ọzọkwa, nyochaa serum maka ihe ịrịba ama nke hemolysis (acha uhie uhie) na turbidity (mmiri ara ehi ma ọ bụ opaque) sitena-ejide ya na ìhè. Jide n'aka na ị ga-enye ụlọ nyocha ahụ ọnụọgụ ọbara akọwapụtara.
6. Gbaa mbọ hụ naA na-akpọ tube nke ọma na ozi niile dị mkpa ma ọ bụ koodu mmanya.
7.Ọ bụrụ na achọghị ihe atụ oyi kpọnwụrụ, ọ dịghị mkpa ịnyefe serum na tube njem plastik.
8.MgbeA na-achọ ọbara oyi kpọnwụrụ, na-ebufe serum mgbe niile (iji pipette nwere ike ịtụfu) n'ime iche, nke akpọrọ nke ọma.tube mbufe rọba Tinye tube ozugbo n'ime friza nke friji, ma gwa yaonye nnọchi anya ọrụ ọkachamara na ị nwere ụdị oyi kpọnwụrụ nke a ga-eburu.Nyefee tube mbufe rọba dị iche iche akpọrọ nke ọma maka ule ọ bụla chọrọ nlele oyi kpọnwụrụ.Ọ gwụla ma egosiputara n'ụzọ ọzọ, enwere ike izipu ihe nlele serum na ụlọ okpomọkụ.
