Nchọpụta Nucleic Acid Ọcha Ọcha
Nkọwa dị mkpirikpi:
A na-eji ya eme ihe maka nchọpụta nucleic acid, a na-emepụta ya kpamkpam n'okpuru ọnọdụ nchacha, nke na-ebelata mmetọ nwere ike ime n'oge usoro mmepụta ma na-ebelata mmetụta nke mmetọ na-ebufe na nnwale.
1. Nnwale olu nke mmiri ara ehi: Olu mmiri ara ehi, ya bụ, ọnụọgụ ọbara a na-adọta, nwere njehie n'ime ± 10%, ma ọ bụghị ya erughị eru.Ọnụ ọgụgụ ọbara na-ezighi ezi bụ nnukwu nsogbu ugbu a.Nke a abụghị naanị na-ebute nsonaazụ nyocha na-ezighi ezi, mana ọ na-ebute mkpọchi na mebie akụrụngwa nyocha.
2. Nnwale ntapu nke akpa: A na-atụba tube mkpokọta ọbara agụụ nwere sodium fluorescein composite solution n'ime mmiri deionized maka nkeji iri isii.N'okpuru isi iyi ọkụ ultraviolet na-efegharị ogologo, ọ dịghị ihe ọ bụla a hụrụ n'okpuru ọhụụ nkịtị n'ime ụlọ gbara ọchịchịrị, nke tozuru oke.Mwepu nke akpa bụ isi ihe kpatara oke ọbara na-ezighi ezi nke tube mkpokọ ọbara dị ugbu a.
3. Nnwale ike nke akpa: a na-edobe akpa ahụ na centrifuge na centrifugal acceleration nke 3000g maka nkeji 10, ọ ruru eru ma ọ bụrụ na ọ naghị agbawa.Ihe siri ike chọrọ ná mba ọzọ bụ: mita 2 n'elu ala, oghere ọbara na-anakọta tube na-ada na kwụ ọtọ na-agbajighị, nke nwere ike igbochi mmebi mberede na tube ule na nkwụsị nke ụdị.
4. Nnwale oghere efu kacha nta: Opekempe kacha nta iji hụ na ọbara agwakọtara nke ọma.Ọnụ ọgụgụ ọbara ewepụtara bụ 0.5ml-5ml,>+25% nke ọbara ewepụtara;ọ bụrụ na ọnụ ọgụgụ ọbara ewepụtara bụ>5ml,>15% nke ọbara ewepụtara.
5. Nnwale ziri ezi nke ihe mgbaze, oke oke solute na ego mgbakwunye ngwọta: njehie kwesịrị ịdị n'ime ± 10% nke osisi ọkọlọtọ akọwapụtara.Nke a bụ nsogbu a na-eleghara anya na nke a na-ahụkarị, ọ bụkwa otu n'ime isi ihe kpatara data ule ezighi ezi.
