Otu eriri mkpụrụ ndụ muclear jel nkewa Tube—Tube CPT

Otu eriri mkpụrụ ndụ muclear cell Separation Tube—Image egosipụtara Tube CPT

Nkọwa dị mkpirikpi:

A na-eji kewapụ monocytes n'ọbara dum.

A na-ejikarị ya maka nchọpụta ọrịa na-alụso ọrịa ọgụ lymphocyte dị ka HLA, nchọpụta mkpụrụ ndụ leukemia fọdụrụnụ na ọgwụgwọ cell dịghịzi.

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Kedu ihe ọ bụ CPT Tube

A na-agbakwunye otu muklear cell gel seperation tube (CPT tube) na hypaque, anticoagulant na gel nkewa.Lymphocytes na mkpụrụ ndụ mononuclear nwere ike kewapụ ya n'ọbara dum site na otu nzọụkwụ centrifugation mgbe ị na-eji gel nkewa pụrụ iche.A na-eji ya eme ihe maka nchọpụta nke ọrụ mgbochi lymphocyte, HLA ma ọ bụ nchọpụta mkpụrụ ndụ leukemia fọdụrụnụ na ọgwụgwọ cell dịghịzi.Ọ na-enye usoro ọkọlọtọ maka mmịpụta otu-nzọụkwụ nke monocytes maka nkwadebe ụdị nyocha ụlọ ọgwụ yana immunotherapy cellular.

Ọrụ ngwaahịa

1) Nha: 13 * 100mm, 16 * 125mm;

2) Mgbakwunye Mpịakọta: 0.1ml, 135usp;

3) Mpịakọta ọbara: 4ml,8ml;

4) Ndụ shelf: ọnwa 24 site na ụbọchị arụpụtara;

5) Nchekwa:Na-echekwa na 18-25℃

NgwaahịaUru

1) Ọ dị mma, nke ziri ezi na nchekwa;

2) Site na Ficoll Hypaque wuru, anticoagulant na gel nkewa, mkpụrụ ndụ mononuclear na-ekewa site na ọbara dum site na otu nzọụkwụ centrifugation.

3) Teknụzụ nkewa cell nke ọma.

4) Nkà na ụzụ nhazi bionic membrane dị n'ime mgbidi;

5) Ọnụ ọgụgụ mgbake nke monocytes karịrị 90%, ịdị ọcha karịa 95%, na ịdị ndụ karịa 99%

Ihe ndị chọrọ nlebara anya

Ihe ndị a kwesịrị iburu n'uche:

1) Mgbe ị na-eme nnwale nke culturing mkpụrụ ndụ, ntị na aseptic ọrụ, igba ogwu na reagents (nkewa ngwọta, ịsa ihe ngwọta, wdg) na ngwá .Ndị ọkachamara kwesịrị ịrụ ọrụ a iji kwe nkwa njikọta nke ọrụ ahụ.

2) Centrifugation okpomọkụ na-ejikarị na ụlọ okpomọkụ (2 ~ 25 ℃).

3) Ọtụtụ mgbe mgbe ị na-ekewa mkpụrụ ndụ mononuclear (PBMC) na Ficoll, ọnụọgụ ọbara ọbara dị ntakịrị, nke nwere ike imetụta izi ezi nke nnwale ahụ.Ya mere, a pụrụ iji lysate mee ihe (ụfọdụ dị mkpa ka a gwọọ ya), na-achịkwa oge lysis ma gbochie imetụta ọrụ nke mkpụrụ ndụ mononuclear.

4) Kpachara anya banyere re-centrifugation nke nwere ike okpukpu abụọ nke dilution.

Nke gara aga: CTAD Nchọpụta Tube

Osote: Ọbara Ọbara Na-acha uhie uhie