Plasma bara ụba nke platelet na-akpali angiogenesis na ụmụ oke nke nwere ike ịkwalite uto ntutu

Platelet-rich plasma (PRP) bụ mkpokọta platelet nke mmadụ na-ahụ maka onwe ya na plasma.Site na mmebi nke granules alpha na platelet, PRP nwere ike zoo ihe dị iche iche na-eto eto, gụnyere platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), hepatocyte growth factor (HGF), na mgbanwe. ihe na-eto eto (TGF), bụ nke edepụtara iji malite ọgwụgwọ ọnya na ịkwalite mmụba na mgbanwe nke mkpụrụ ndụ endothelial na pericytes n'ime endothelial pulite.

A kọwo ọrụ PRP maka ọgwụgwọ ntutu isi n'ọtụtụ nyocha ndị na-adịbeghị anya.Uebel et al.achọpụtala na ihe na-eto eto plasma platelet na-abawanye mkpụrụ nke nkeji follicular na ịwa isi nkwọcha nwoke.Ọrụ na-adịbeghị anya egosila na PRP na-abawanye ụba nke mkpụrụ ndụ papilla dermal ma mee ka mgbanwe telogen-to-anagen ngwa ngwa site na iji ụdị vivo na in vitro.Nnyocha ọzọ egosila na PRP na-akwalite nhazigharị ntutu isi ma na-ebelata oge nhazi ntutu.

Ma PRP na platelet-ogbenye plasma (PPP) gụnyere njuju nke protein coagulation.N'ime ọmụmụ ihe ugbu a, a na-enyocha mmetụta PRP na PPP na ntutu isi na C57BL / 6 ụmụ oke.Echiche ahụ bụ na PRP nwere mmetụta dị mma na ogologo ntutu isi na mmụba nke ọnụ ọgụgụ ntutu isi.

Ụmụ anụmanụ na-anwale

Enwetara 50 ahụ ike C57BL / 6 ụmụ oke nwoke (izu isii, 20 ± 2 g) sitere na Center of Laboratory Animals, Hangzhou Normal University (Hangzhou, China).A na-enye anụmanụ nri otu nri ma na-edobe ya na gburugburu ebe niile n'okpuru 12:12-h ìhè-ọchịchịrị okirikiri.Mgbe izu 1 gachara, a na-ekewa ụmụ oke n'ụzọ atọ: otu PRP (n = 10), otu PPP (n = 10), na otu njikwa (n = 10).

Ndị kọmitii ụkpụrụ omume ụlọ ọrụ nke nyocha anụmanụ kwadoro ụkpụrụ ọmụmụ ahụ n'okpuru Iwu nke nyocha anụmanụ na iwu iwu na China.

Nleta ogologo ntutu isi

Na 8, 13, na 18 ụbọchị ka agbachara ikpeazụ, ntutu 10 na òké ọ bụla ka ahọpụtara na-enweghị usoro na mpaghara ebumnuche.A na-eji microscope eletrọn were were tụọ ogologo ntutu isi n'ubi atọ, ma gosipụta nkezi ha dị ka milimita.Ewepụla ntutu elongated ma ọ bụ mebiri emebi.

Hematoxylin na eosin (HE).

E wepụrụ ihe nlele akpụkpọ anụ ahụ n'ime ụbọchị iri na asatọ ka ịgbanye ọgwụ mgbochi nke atọ.Mgbe ahụ, a na-edozi ihe nlele na 10% na-anọpụ iche nke formalin, agbakwunyere na paraffin, ma bee n'ime 4 μm.A na-eme akụkụ ndị ahụ maka awa 4 maka deparaffinization na 65 Celsius C, tinye ya na ethanol gradient, wee tinye ya na hematoxylin maka 5 min.Mgbe e kechara ọdịiche dị na 1% hydrochloric acid mmanya, a na-etinye akụkụ ndị ahụ na mmiri amonia, na-emetọ ya na eosin, ma kpochaa ya na mmiri mmiri.N'ikpeazụ, a na-eji ethanol gradient kpochapu akụkụ ahụ, kpochaa ya na xylene, tinye ya na resin na-anọpụ iche, wee hụ na-eji obere microscopy (Olympus, Tokyo, Japan).