Tube mkpokọta mkpochapụ ọbara isi awọ
Nkọwa dị mkpirikpi:
Potassium oxalate/sodium fluoride okpu isi awọ.Sodium fluoride bụ ọgwụ mgbochi na-adịghị ike.A na-ejikarị ya na potassium oxalate ma ọ bụ sodium ethiodate.Oke ya bụ akụkụ 1 nke sodium fluoride na akụkụ 3 nke potassium oxalate.4mg nke ngwakọta a nwere ike ime ka 1ml nke ọbara ghara ịchịkọta ma gbochie glycolysis n'ime ụbọchị 23.Ọ bụ ihe nchekwa dị mma maka ikpebi glucose ọbara, enweghịkwa ike iji ya maka mkpebi urea site na usoro urease, ma ọ bụ maka mkpebi alkaline phosphatase na amylase.Akwadoro maka nyocha shuga ọbara.
Ebumnobi: Glucose bụ otu n'ime nyocha a na-atụkarị na ụlọ nyocha.Nnyocha kacha nso nso a gbasara nkwụsi ike glucose na-akwado na tube sodium fluoride/potassium oxalate (NaF/KOx) dị anya na ọkọlọtọ ọla edo.Ọtụtụ ụlọ ọrụ atụnyere tubes citrate dị ka ụdị tube kacha amasị.Greiner ewebatala tube nke akọwapụtara glucose (Glucomedics) nwere NaF/KOx, citrate na EDTA iji belata glycolysis.Ebumnuche bụ ikpebi tube ga-adabara maka nleba anya glucose ziri ezi na ụlọ nyocha oge niile.
Nhazi na ụzọ: Usoro ọmụmụ gụnyere nnwale atọ: (a) ntụnyere ndị sonyere na-eji plasma lithium heparin dị ka ihe atụ atụnyere;(b) ọmụmụ nkwụsi ike (0, 1, 2 na 4 h);na (c) obere njupụta maka citrate na tubes Glucomedics.
Nsonaazụ: Nnyocha ntụle ndị ọrịa nke lithium heparin plasma gosiri na EDTA, NaF/KOx, na ma citrate na Glucomedics ma ọ bụrụ na edoziziri maka ihe dilutional rụpụtara nsonaazụ nnabata.Ọmụmụ nkwụsi ike ruo awa 4 gosiri na tube Glucomedics kacha dị irè n'igbochi mgbanwe n'ụlọ ọgwụ na mkpokọta glucose n'ime ụlọ.Ma citrate na Glucomedics kwesịrị ijupụta n'ime 0.5 ml nke olu njuju akwadoro maka nsonaazụ anabatara.
Nchikota: Glucomedics tube kacha mma maka ibelata glycolysis.Ọganihu ndị ọzọ na ya (iji ihe dilutional ziri ezi na mgbakwunye nke gel separator) ga-eme ka tube a bụrụ akara ngosi maka ntule ziri ezi, nchọpụta kacha mma na mkpebi nlekọta ndị ọrịa.
