Gel Yellow ọbara mkpokọta tube
Nkọwa dị mkpirikpi:
Maka nchọpụta biochemical, nnwale immunological, wdg, adịghị akwadoro maka mpụta mmewere.
Nkà na ụzụ dị elu dị elu na-eme ka ọ dị mma nke ọma, nchekwa okpomọkụ dị ala, yana nchekwa oyi kpọnwụrụ nke ụdị ga-ekwe omume.
A na-eji tubes nchịkọta ọbara nkewa nkewa mee ihe na ụlọ nyocha ụlọ ọgwụ.Kewapụ gel nwere ike ịmepụta oyi akwa dị n'etiti ihe ndị dị na cell na serum (plasma), na-egbochi mgbanwe ihe onwunwe n'etiti mkpụrụ ndụ ọbara na serum (plasma), ma hụ na nkwụsi ike nke serum (plasma) components n'ime oge ụfọdụ.Ikewa gluu bụ tumadi mejupụtara silicone roba, macromolecular hydrocarbons, hydrophobic gluu, wdg Dị ka a polymer ihe, ọ bụ insoluble na mmiri na inert.Ọ bụ mmiri mmiri viscous thixotropic nke nwere njupụta nke 1.04-1.05 mmol / N'etiti L, ọ nwere uru nke nguzogide oxidation, oke okpomọkụ na-eguzogide, obere okpomọkụ na-eguzogide, na ezigbo ikuku.Njupụta nke serum bụ 1.026-1.031 mmol / L, na hematocrit bụ 1.090-1.095.N'ihi ike ndọda a kapịrị ọnụ, gel nkewa dị n'etiti serum na sel ọbara, yabụ n'ọnọdụ nkịtị, ọbara ga-apụta n'usoro mgbe centrifugation gasịrị.Serum, gel nkewa, na sel ọbara 3 ala.
A na-enwekarị ụdị abụọ nkewapụ mkpokọta ọbara gel nke a na-ejikarị na ụlọ nyocha: serum separation gel procoagulation tube na plasma separation gel anticoagulation tube.The serum separation gel procoagulation tube bụ ịgbakwunye coagulant na tube mkpokọta ọbara na-ebelata oge coagulation ọbara, nweta serum ngwa ngwa, na-akọkwa arụpụtaghị n'ime obere oge.Igwe mkpokọ ọbara iko adịghị mkpa ịgbakwunye coagulant, na ọbara ịkpọtụrụ mgbidi tube iko ga-akpalite coagulation.Agbanyeghị, mgbe ihe na-akpata coagulation XI na XII batara na tubes na-anakọta ọbara rọba, ikike ịgbanye ha adịghị ike nke ukwuu, ọ dị mkpa ka etinyekwa coagulant iji belata oge coagulation.A na-efesa tube nkewa nkewa gel anticoagulation tube na anticoagulants dị ka lithium heparin na mgbidi dị n'ime nke mkpokọta ọbara gel nkewa iji gboo mkpa nke nnwale mberede biochemical nke plasma ngwa ngwa.
N'ime ngwa bara uru, a na-ezutekarị na mmetụta nkewa nke nkewa nkewa gel na-anakọta tube adịghị mma, dịka ọmụmaatụ: na ụfọdụ tubes roba nkewa, ọ nwere ike ịhụ na nkewa nkewa jel ma ọ bụ ụmụ irighiri mmanụ na-ese n'elu n'elu. serum ma ọ bụ kwụsịtụrụ na serum;nkewa gel oyi akwa sere n'elu serum oyi akwa.n'elu wdg. Nkewa gels nwekwara ike igbochi ụfọdụ nsonaazụ ule.Na ngalaba anyị, achọpụtara na otu ụdị reagents na serum separation gel accelerating tube na-emeghachi omume n'otu n'otu n'oge nchọpụta HBSAg nke sistemụ nchọpụta Abbott i2000SR, na-ebute nsonaazụ dị mma.
Akwụkwọ a na-enyochakarị site na akụkụ abụọ, ya bụ, ihe kpatara mmetụta nkewa na-adịghị mma nke gel nkewa, na mmetụta nke ntinye nke gel na-ekewapụ na nha.
1. Usoro nkewapụ serum na plasma site na-ekewa gel Separating gel bụ thixotropic mucocolloid nke mejupụtara ogige hydrophobic organic na silica ntụ ntụ.Ọdịdị ahụ nwere ọnụ ọgụgụ buru ibu nke njikọ hydrogen.Ịdị adị nke njikọ hydrogen bụ ihe ndabere kemịkal nke thixotropy nke gel na-ekewa..A na-edobe ike ndọda nke gel nkewa na 1.05, ike ndọda nke ihe mejupụtara ọbara bụ ihe dịka 1.02, na ike ndọda nke ihe mejupụtara ọbara bụ ihe dịka 1.08.Mgbe a na-ekewa gel na-ekewapụ na ọbara na-agbakọta (ma ọ bụ ọbara na-eme ka ọ ghara ikpochapụ) n'ime otu ule nyocha ahụ, N'ihi ike centrifugal na-etinye na gel nkewa, a na-agbaji usoro netwọk hydrogen bond n'ime usoro dị ka agbụ, na nkewa. jel na-aghọ mmiri mmiri dị ala.N'ihi ike ndọda dị iche iche, a na-atụgharị gel nkewa ahụ wee gbanwee ka ọ mepụta okpukpu atọ nke ọbara ọgbụgba (ọbara na-egbochi ọbara dum) / gel / serum (plasma).Mgbe centrifuge kwụsịrị ịgbagharị ma tụfuo ike centrifugal, ihe ndị a yinye na gel nkewa na-etolite usoro netwọk ọzọ site na agbụ hydrogen, weghachi ọnọdụ gel viscosity mbụ dị elu, wee mepụta oyi akwa dị n'etiti serum (plasma) na mkpụkọ ọbara (anticoagulated). ọbara dum)..
2. Ihe kpatara mmetụta nkewa adịghị mma nke ikewa gel
2.1 Ikewa Gel Ogo Ike ike dị iche iche nke gel na-ekewa bụ n'etiti nke serum (plasma) na sel ọbara, nke bụ ihe ndabere anụ ahụ maka ngbanwe nke gel na-ekewapụ na nkewa nke serum (plasma).Ọ bụrụ na ịdị mma nke gel nkewa nke tube mkpokọta ọbara adịghị mma na ike ndọda a kapịrị ọnụ adịghị emezu ihe achọrọ, ọ ga-abụrịrị na ọ ga-emetụta mmetụta nkewapụ serum (plasma), na ihe omume na nkewa nkewa na serum (plasma) bụ. ejikọrọ nwere ike ime.
2.2 coagulation ọbara na-ezughị ezu Mgbe centrifugation gasịrị, mgbe ụfọdụ akụkụ gel nkewa na serum na mkpụkọ ọbara anaghị ekewa kpamkpam, fibrin filaments na-apụta na serum.Ihe kpatara ya na-abụkarị na ọbara anaghị agbakọta nke ọma tupu centrifugation.Mkpuchi ọbara na-ezughị ezu nwere ike ime ka agwakọta fibrin na oyi akwa dịpụrụ adịpụ.A ghaghị iji tube separation rubber serum mee ihe n'ụzọ ziri ezi dị ka ntuziaka ahụ si dị, a pụkwara ịkwadebe ọbara ahụ site na centrifugation mgbe a na-ejikọta ọbara kpamkpam (n'ozuzu, tube plastik nke nwere coagulant kwesịrị ka edobe ya nke ọma maka ihe dị ka nkeji 30, na ọbara ahụ). tube collection na-enweghị coagulant kwesịrị ka edobe ya kwụ ọtọ maka nkeji 60-90).ụdị serum dị elu.
2.3 Centrifugation okpomọkụ okpomọkụ centrifugation nwere mmetụta dị ịrịba ama na mmetụta nke ikewapụ serum na nkewa gel tube.Ọbara ahụ doro anya na inert kewara gel accelerated coagulation tube kewapụrụ site na nkịtị centrifuge na ọnụ ụlọ okpomọkụ, ma mmanu beads nke dị iche iche nha pụtara na 15% ka 20% nke samples.N'aka nke ọzọ, ọ dịghị bead mmanu a hụrụ na serum kewapụrụ na test tube centrifuge site na obere okpomọkụ centrifuge.Mgbe ọnọdụ okpomọkụ karịrị nchekwa nchekwa achọrọ maka gel nkewa, gel inert ga-agbaze na serum.Ọ bụghị naanị na ọ ga-egbochi ma metọọ agịga nlele na iko mmeghachi omume nke ihe nyocha biochemical, kamakwa ọ ga-enwe mmetụta dịtụ ukwuu na nsonaazụ nha biochemical.
